HPAEC-PAD Dionex BioLC system (old system)

This HPLC system is mostly used for analytical quantification of carbohydrate (neutral sugars, sugar-phosphate and sugar-polymers) derived from biological samples. Separation occurs usually via anion exchange chromatography at high pH and is detected by a pulsed amperometric detector. Most carbohydrates are detectable quantitatively in a range from 1µM to 1mM with a detection limit of 100pmol (in 100µl sample). Different applications may require other columns / eluents and individual set up.

Further, semi-preparative applications for purification are possible. The attached fraction collector allows automated collection of substances.

Equipment & columns

ICS3000 pump (max. 5ml/min flow)

Auto sampler / AS50 (max 100 samples)

Pulsed amperometric detection (PAD)-detector / ED50

Fraction collector / Foxy Jr. (max. 144 samples each 12ml)

Analytical column

DownloadCarboPac / PA20 (PDF, 703 KB) (max. 0.5ml/min flow)

DownloadCarboPac / PA200 (PDF, 353 KB) (max. 0.5ml/min flow)

DownloadCarboPac / PA100 (PDF, 381 KB) (max. 1.0ml/min flow)

Semi-Preperative column

CarboPac / PA100 (best 2ml/min flow)

Trap column

AminoTrap (max. 0.5ml/min flow)

Other columns

DownloadIonPac / AS11 (PDF, 504 KB) (max. 2ml/min flow)

Software

Chromeleon 7 (installed in LFW D51 & D36)

Usage

Neutral sugars (PA20, PA100)

Longer polysaccharides (PA100, PA200)

Sugar-phosphates & Nucleotide-sugars, Nucleotide (PA20)

Organic acids & inorganic anions (AS11)

Established measurement: Glucose / Fructose / Sucrose / Maltose / Maltotriose / Raffinose / Stachiose/ Glucose-polymers

Measurable / not tested on biological samples: Glucose-1-phiosphate / Glucose-6-phosphate / Fructose-6-phosphate / ADP-Glucose / UDP-Glucose

Measurable / not established method: Several sugar phosphate / Organic Acids / Inorganic anions / Phospho-glucans